ºðºì²ÜÆ äºî²Î²Ü вزÈê²ð²ÜÆ ¶Æî²Î²Ü îºÔºÎ²¶Æð Ó×ÅÍÛÅ ÇÀÏÈÑÊÈ ÅÐÅÂÀÍÑÊÎÃÎ ÃÎÑÓÄÀÐÑÒÂÅÍ ÍÎÃÎ ÓÍÈÂÅÐ ÑÈÒÅÒÀ ´Ý³Ï³Ý ·ÇïáõÃÛáõÝÝ»ñ êè 1, 2007 Åñòåñòâåííûå íàó- Ȼɢ ɨ ɥ ɨ ɝ ɢ ɹ ɍȾɄ 612.014.4.083.36 Ʉ. ɋ. ɆȺɊȽȺɊəɇ ɋɊȺȼɇɂɌ ȿɅɖɇɕɃ ȺɇȺɅɂɁ ȽȿɇɈɌ ɈɄɋɂɑȿɋɄɂɏ ɗɎɎȿɄɌ Ɉȼ ɉɈɊɎɂɊɂɇȺ FeT OBut4PyP ȼ ɄɅȿɌ ɈɑɇɈɃ ɂ ɇȿɄɅȿɌ ɈɑɇɈɃ ɋɂɋɌ ȿɆȺɏ ɆȿɌɈȾɈɆ ȾɇɄ-ɄɈɆȿɌ Ɇɟɬɨɞɨɦ ȾɇɄ-ɤɨɦɟɬ ɛɵɥɚ ɢɡɭɱɟɧɚ ɝɟɧɨɬɨɤɫɢɱɧɨɫɬɶ ɩɨɪɮɢɪɢɧɚ FeTOBut4PyP ɜ ɤɥɟɬɨɱɧɨɣ ɢ ɧɟɤɥɟɬɨɱɧɨɣ ɫɢɫɬɟɦɚɯ. ȼɵɹɜɥɟɧɧɵɟ ɪɚɡɥɢɱɢɹ ɜ ɱɭɜɫɬɜɢɬɟɥɶɧɨɫɬɢ ɞɜɭɯ ɦɨɞɟɥɟɣ ɨɬɪɚɠɚɸɬ ɪɚɡɧɵɟ ɭɪɨɜɧɢ ɢɯ ɨɪɝɚɧɢɡɚɰɢɢ. ɉɪɢɦɟɧɟɧɧɵɣ ɩɨɞɯɨɞ ɦɨɠɟɬ ɛɵɬɶ ɪɟɤɨɦɟɧɞɨɜɚɧ ɞɥɹ ɨɰɟɧɤɢ ɝɟɧɨɬɨɤɫɢɱɟɫɤɢɯ ɷɮɮɟɤɬɨɜ ɪɚɡɧɵɯ ɫɨɟɞɢɧɟɧɢɣ, ɚ ɬɚɤɠɟ ɞɥɹ ɫɪɚɜɧɢɬɟɥɶɧɨɝɨ ɚɧɚɥɢɡɚ ɪɨɥɢ ɤɥɟɬɨɱɧɵɯ ɦɟɯɚɧɢɡɦɨɜ ɜ ɮɨɪɦɢɪɨɜɚɧɢɢ ɩɨɜɪɟɠɞɟɧɢɣ ȾɇɄ. ȼɜɟ ɞɟ ɧɢɟ . ȼ ɩɨɫɥɟɞɧɢɟ ɝɨɞɵ ɩɨɪɮɢɪɢɧɵ ɩɪɢɨɛɪɟɥɢ ɛɨɥɶɲɨɟ ɩɪɢɦɟɧɟɧɢɟ ɜ ɮɚɪɦɚɤɨɥɨɝɢɢ, ɬ.ɤ. ɨɧɢ ɲɢɪɨɤɨ ɪɚɫɩɪɨɫɬɪɚɧɟɧɵ ɜ ɩɪɢɪɨɞɟ (ɯɥɨɪɨɮɢɥɥ, ɝɟɦ, ɛɚɤɬɟɪɢɨɯɥɨɪɨɮɢɥɥ [1], ɩɨɪɮɢɪɢɧɵ ɧɟɮɬɢ ɢ ɞɪɭɝɢɯ ɦɢɧɟɪɚɥɶɧɵɯ ɩɨɪɨɞ). Ɉɫɨɛɟɧɧɨɫɬɶɸ ɩɨɪɮɢɪɢɧɨɜ ɹɜɥɹɟɬɫɹ ɢɯ ɫɩɨɫɨɛɧɨɫɬɶ ɫɟɥɟɤɬɢɜɧɨ ɧɚɤɚɩɥɢɜɚɬɶɫɹ ɜ ɨɩɭɯɨɥɟɜɵɯ ɬɤɚɧɹɯ [2]. ɉɪɢɦɟɧɟɧɢɟ ɩɨɪɮɢɪɢɧɨɜ ɜ ɤɥɢɧɢɱɟɫɤɨɣ ɩɪɚɤɬɢɤɟ ɫɬɢɦɭɥɢɪɨɜɚɥɨ ɩɨɢɫɤ ɢɯ ɧɨɜɵɯ ɩɪɨɢɡɜɨɞɧɵɯ ɫ ɭɥɭɱɲɟɧɧɵɦɢ ɫɜɨɣɫɬɜɚɦɢ [3]. ȼ ɫɜɹɡɢ ɫ ɬɟɦ, ɱɬɨ FeT OBut4PyP (Fe-ɩɪɨɢɡɜɨɞɧɨɟ ɬɟɬɪɚɨɤɫɢɛɭɬɢɥɩɢɪɢɞɢɥ ɩɨɪɮɢɪɢɧɚ) ɹɜɥɹɟɬɫɹ ɧɨɜɵɦ ɩɨɪɮɢɪɢɧɨɦ, ɦɟɯɚɧɢɡɦɵ ɟɝɨ ɞɟɣɫɬɜɢɹ ɧɚ ɤɥɟɬɤɭ ɢ, ɜ ɬɨɦ ɱɢɫɥɟ, ɧɚ ɤɥɟɬɨɱɧɭɸ ȾɇɄ ɪɚɧɟɟ ɧɟ ɨɩɢɫɚɧɵ. ɋɨɝɥɚɫɧɨ ɥɢɬɟɪɚɬɭɪɧɵɦ ɞɚɧɧɵɦ, ɩɨɪɮɢɪɢɧɵ, ɢɦɟɸɳɢɟ ɩɪɨɫɬɪɚɧɫɬɜɟɧɧɭɸ ɫɬɪɭɤɬɭɪɭ, ɜɧɟɲɧɟ ɫɜɹɡɵɜɚɸɬɫɹ ɫ ȾɇɄ ɩɪɟɢɦɭɳɟɫɬɜɟɧɧɨ ɜ ȺɌ -ɛɨɝɚɬɵɯ ɭɱɚɫɬɤɚɯ [4]. Ⱦɥɹ ɢɡɭɱɟɧɢɹ ɩɨɬɟɧɰɢɚɥɶɧɨɣ ɝɟɧɨɬɨɤɫɢɱɧɨɫɬɢ FeT OBut4PyP ɢ ɪɨɥɢ ɤɥɟɬɨɱɧɵɯ ɦɟɯɚɧɢɡɦɨɜ ɜ ɮɨɪɦɢɪɨɜɚɧɢɢ ɢɧɞɭɰɢɪɨɜɚɧɧɵɯ ɩɨɪɮɢɪɢɧɨɦ ɩɨɜɪɟɠɞɟɧɢɣ ȾɇɄ ɩɪɢɦɟɧɹɥɢ ɦɟɬɨɞ ȾɇɄ-ɤɨɦɟɬ (ɝɟɥɶ-ɷɥɟɤɬɪɨɮɨɪɟɡ ɨɬɞɟɥɶɧɵɯ ɤɥɟɬɨɤ), ɩɨɡɜɨɥɹɸɳɢɣ ɛɵɫɬɪɨ ɢ ɷɮɮɟɤɬɢɜɧɨ ɨɰɟɧɢɜɚɬɶ ɭɪɨɜɧɢ ɪɚɡɪɵɜɨɜ ȾɇɄ ɤɚɤ ɜ ɤɥɟɬɨɱɧɨɣ, ɬɚɤ ɢ ɜ ɧɟɤɥɟɬɨɱɧɨɣ ɫɢɫɬɟɦɚɯ [5]. ɉɪɢ ɫɬɚɧɞɚɪɬɧɨɦ ɦɟɬɨɞɟ ȾɇɄ-ɤɨɦɟɬ (ɤɥɟɬɨɱɧɚɹ ɫɢɫɬɟɦɚ) ɩɨɝɪɭɠɟɧɧɵɟ ɜ ɥɟɝɤɨɩɥɚɜɤɭɸ ɚɝɚɪɨɡɭ ɤɥɟɬɤɢ ɧɚɧɨɫɹɬɫɹ ɧɚ ɩɪɟɞɦɟɬɧɵɟ ɫɬɟɤɥɚ, ɩɪɟɞɜɚɪɢɬɟɥɶɧɨ ɩɨɤɪɵɬɵɟ ɫɥɨɟɦ ɚɝɚɪɨɡɧɨɝɨ ɝɟɥɹ [6]. Ʉɥɟɬɤɢ ɩɪɢ ɩɨɦɨɳɢ ɞɟɬɟɪɝɟɧɬɨɜ ɢ ɫɨɥɟɣ ɜɵɫɨɤɨɣ ɤɨɧɰɟɧɬɪɚɰɢɢ ɥɢɡɢɪɭɸɬɫɹ ɩɪɢ ɪɇ 10 (ɩɪɢ ɷɬɨɦ 72 ɪɚɡɪɭɲɚɸɬɫɹ 95% ɩɪɨɬɟɢɧɨɜ ɢ ɤɥɟɬɨɱɧɵɯ ɦɟɦɛɪɚɧ). ɉɨɫɥɟ ɥɢɡɢɫɚ ɢ ɷɥɟɤɬɪɨɮɨɪɟɡɚ ɡɚɤɥɸɱɟɧɧɵɯ ɜ ɚɝɚɪɨɡɧɵɣ ɫɥɨɣ ɷɭɤɚɪɢɨɬɢɱɟɫɤɢɯ ɤɥɟɬɨɤ ɩɨɜɪɟɠɞɟɧɧɚɹ ȾɇɄ ɦɢɝɪɢɪɭɟɬ ɜ ɷɥɟɤɬɪɢɱɟɫɤɨɦ ɩɨɥɟ ɩɨ ɧɚɩɪɚɜɥɟɧɢɸ ɤ ɚɧɨɞɭ, ɨɛɪɚɡɭɹ ɫɬɪɭɤɬɭɪɭ, ɩɨɯɨɠɭɸ ɧɚ ɤɨɦɟɬɭ ɫ «ɝɨɥɨɜɨɣ» ɢɡ ɤɥɭɛɤɚ ȾɇɄ ɢ «ɯɜɨɫɬɨɦ» ɢɡ ɦɢɝɪɢɪɨɜɚɜɲɢɯ ȾɇɄ. ɉɪɢ ɷɬɨɦ ɩɨ ɫɬɟɩɟɧɢ ɦɢɝɪɚɰɢɢ ȾɇɄ ɢɡ ɹɞɪɚ ɦɨɠɧɨ ɫɭɞɢɬɶ ɨ ɫɬɟɩɟɧɢ ɟɟ ɩɨɜɪɟɠɞɟɧɢɹ. Ⱦɪɭɝɚɹ ɜɟɪɫɢɹ ɦɟɬɨɞɚ ȾɇɄ-ɤɨɦɟɬ ɩɨɡɜɨɥɹɟɬ ɨɰɟɧɢɜɚɬɶ ɭɪɨɜɟɧɶ ɩɨɜɪɟɠɞɟɧɢɣ ȾɇɄ ɜ ɧɟɤɥɟɬɨɱɧɨɣ ɫɢɫɬɟɦɟ. ɇɟɨɛɯɨɞɢɦɵɦ ɷɬɚɩɨɦ ɩɪɟɞɨɛɪɚɛɨɬɤɢ ɢɡɭɱɚɟɦɵɦɢ ɜɟɳɟɫɬɜɚɦɢ ɩɪɢ ɷɬɨɦ ɜɚɪɢɚɧɬɟ ɦɟɬɨɞɚ ɹɜɥɹɟɬɫɹ ɥɢɡɢɫ ɤɥɟɬɨɱɧɨɣ ɢ ɹɞɟɪɧɨɣ ɦɟɦɛɪɚɧ ɢ ɱɚɫɬɢɱɧɨɟ ɪɚɡɪɭɲɟɧɢɟ ɜɧɭɬɪɢɹɞɟɪɧɵɯ ɛɟɥɤɨɜ, ɭɱɚɫɬɜɭɸɳɢɯ ɜ ɭɩɚɤɨɜɤɟ ɯɪɨɦɚɬɢɧɚ, ɫ ɰɟɥɶɸ ɨɫɜɨɛɨɠɞɟɧɢɹ ȾɇɄ ɞɥɹ ɨɬɧɨɫɢɬɟɥɶɧɨ ɫɜɨɛɨɞɧɨɣ ɦɢɝɪɚɰɢɢ ɜ ɝɟɥɟ ɩɪɢ ɷɥɟɤɬɪɨɮɨɪɟɡɟ [7]. ɉɪɢ ɷɬɨɦ ɞɟɣɫɬɜɢɟ ɜɟɳɟɫɬɜɚ ɧɚ ɤɥɟɬɤɢ ɢɡɭɱɚɟɬɫɹ ɩɨɫɥɟ ɩɪɨɰɟɞɭɪɵ ɥɢɡɢɫɚ. ɐɟɥɶɸ ɧɚɲɟɝɨ ɢɫɫɥɟɞɨɜɚɧɢɹ ɛɵɥɨ ɫɪɚɜɧɢɬɟɥɶɧɨɟ ɢɡɭɱɟɧɢɟ ɝɟɧɨɬɨɤɫɢɱɧɨɫɬɢ ɧɨɜɨɝɨ ɩɨɪɮɢɪɢɧɚ FeT OBut4PyP ɜ ɥɟɣɤɨɰɢɬɚɯ ɤɪɨɜɢ ɱɟɥɨɜɟɤɚ ɦɟɬɨɞɨɦ ȾɇɄ-ɤɨɦɟɬ ɜ ɤɥɟɬɨɱɧɨɣ ɢ ɧɟɤɥɟɬɨɱɧɨɣ ɫɢɫɬɟɦɚɯ. Ɇɚɬɟ ɪɢɚɥ ɢ ɦɟɬɨɞɢɤɚ. Ɉɛɪɚɛɨɬɤɚ ɤɥɟɬɨɤ. Ⱦɥɹ ɩɪɨɜɟɞɟɧɢɹ ɢɫɫɥɟɞɨɜɚɧɢɹ ɧɟɩɨɫɪɟɞɫɬɜɟɧɧɨ ɩɟɪɟɞ ɷɤɫɩɟɪɢɦɟɧɬɨɦ ɛɪɚɥɢ ɝɟɩɚɪɢɧɢɡɢɪɨɜɚɧɧɭɸ ɤɚɩɢɥɥɹɪɧɭɸ ɤɪɨɜɶ ɭ ɡɞɨɪɨɜɵɯ ɞɨɧɨɪɨɜ ɜ ɜɨɡɪɚɫɬɟ ɨɬ 20 ɞɨ 25 ɥɟɬ. ɏɚɪɚɤɬɟɪɢɫɬɢɤɚ FeTOBut4PyP. ɗɬɨ ɧɨɜɨɟ ɫɨɟɞɢɧɟɧɢɟ, ɫɢɧɬɟɡɢɪɨɜɚɧɧɨɟ ɧɚ ɤɚɮɟɞɪɟ ɮɚɪɦɚɤɨɥɨɝɢɱɟɫɤɨɣ ɯɢɦɢɢ ȿɪɟɜɚɧɫɤɨɝɨ ɝɨɫɭɞɚɪɫɬɜɟɧɧɨɝɨ ɦɟɞɢɰɢɧɫɤɨɝɨ ɭɧɢɜɟɪɫɢɬɟɬɚ. FeT OBut4PyP – ɬɟɦɧɨ-ɜɢɲɧɟɜɵɣ ɤɪɢɫɬɚɥɥɢɱɟɫɤɢɣ ɩɨɪɨɲɨɤ ɫɨ ɫɥɚɛɵɦ ɫɩɟɰɢɮɢɱɟɫɤɢɦ ɡɚɩɚɯɨɦ, ɯɨɪɨɲɨ ɪɚɫɬɜɨɪɢɦɵɣ ɜ ɜɨɞɟ. ɋɬɚɧɞɚɪɬɧɵɣ ɦɟɬɨɞ ȾɇɄ-ɤɨɦɟɬ (ɤɥɟɬɨɱɧɚɹ ɫɢɫɬɟɦɚ). ȼ ɪɚɛɨɬɟ ɢɫɩɨɥɶɡɨɜɚɥɢ ɳɟɥɨɱɧɭɸ ɜɟɪɫɢɸ ɦɟɬɨɞɚ ɤɨɦɟɬ, ɩɪɟɞɥɨɠɟɧɧɭɸ ɉ. ɋɢɧɯɨɦ ɢ ɫɨɚɜɬɨɪɚɦɢ [8] ɫ ɧɟɛɨɥɶɲɢɦɢ ɦɨɞɢɮɢɤɚɰɢɹɦɢ Ʉɢɮɮɟ ɢ ɫɨɚɜɬɨɪɨɜ [7]. ɇɚ ɩɪɟɞɦɟɬɧɵɟ ɫɬɟɤɥɚ ɧɚɧɨɫɢɥɢɫɶ ɞɜɚ ɚɝɚɪɨɡɧɵɯ ɫɥɨɹ ɜ ɫɥɟɞɭɸɳɟɣ ɩɨɫɥɟɞɨɜɚɬɟɥɶɧɨɫɬɢ: 0 x 1%-ɵɣ ɪɚɫɬɜɨɪ ɚɝɚɪɨɡɵ ɫ ɩɨɫɥɟɞɭɸɳɟɣ ɢɧɤɭɛɚɰɢɟɣ ɩɪɢ 37 ɋ ɧɚ ɧɨɱɶ; x ɫɦɟɫɶ 10 ɦɤɥ ɰɟɥɶɧɨɣ ɤɪɨɜɢ ɱɟɥɨɜɟɤɚ ɫ 90 ɦɤɥ 0,5%-ɝɨ ɪɚɫɬɜɨɪɚ ɚɝɚɪɨɡɵ (LMA), ɢɦɟɸɳɟɣ ɧɢɡɤɭɸ ɬɟɦɩɟɪɚɬɭɪɭ ɩɥɚɜɥɟɧɢɹ. ɉɨɫɥɟ ɧɚɧɟɫɟɧɢɹ ɜɬɨɪɨɝɨ ɫɥɨɹ ɩɪɟɩɚɪɚɬɵ ɩɨɦɟɳɚɥɢɫɶ ɜ ɯɨɥɨɞɢɥɶɧɢɤ ɞɨ ɨɛɪɚɡɨɜɚɧɢɹ ɝɟɥɹ (~10 ɦɢɧ). Ʉɥɟɬɤɢ ɤɪɨɜɢ ɩɨɫɥɟ ɡɚɤɥɸɱɟɧɢɹ ɜ ɚɝɚɪɨɡɧɵɣ ɫɥɨɣ ɨɛɪɚɛɚɬɵɜɚɥɢ ɧɚ ɩɪɟɩɚɪɚɬɟ ɩɨɪɮɢɪɢɧɨɦ FeT OBut4PyP ɜ ɤɨɧɰɟɧɬɪɚɰɢɹɯ 10 -7 , 10 -6 , 10 -5 M. Ɋɚɫɬɜɨɪɵ ɩɨɪɮɢɪɢɧɚ ɪɚɫɤɚɩɵɜɚɥɢ ɧɟɩɨɫɪɟɞɫɬɜɟɧɧɨ ɧɚ ɩɪɟɞɦɟɬɧɵɟ ɫɬɟɤɥɚ, ɱɬɨɛɵ ɫɨɡɞɚɬɶ ɬɟ ɠɟ ɷɤɫɩɟɪɢɦɟɧɬɚɥɶɧɵɟ ɭɫɥɨɜɢɹ, ɱɬɨ ɢ ɩɪɢ ɦɟɬɨɞɟ ɫ ɧɟɤɥɟɬɨɱɧɨɣ ɫɢɫɬɟɦɨɣ. Ɂɚɬɟɦ ɩɪɟɩɚɪɚɬɵ ɢɧɤɭɛɢɪɨɜɚɥɢ ɜ ɬɟɱɟɧɢɟ 2 ɱɚɫɨɜ ɩɪɢ 370 C ɜɨ ɜɥɚɠɧɨɣ ɤɚɦɟɪɟ. Ʉɨɧɬɪɨɥɶɧɵɟ ɜɚɪɢɚɧɬɵ ɨɞɧɨɜɪɟɦɟɧɧɨ ɨɛɪɚɛɚɬɵɜɚɥɢ ɛɭɮɟɪɨɦ 0,4 Ɇ Tris, ɪɇ 7,5. ɉɨɫɥɟ ɢɧɤɭɛɚɰɢɢ ɫ ɩɨɪɮɢɪɢɧɨɦ ɥɢɦɮɨɰɢɬɵ ɧɚ 1 ɱɚɫ ɩɪɢ 40 ɋ ɩɨɦɟɳɚɥɢ ɜ ɥɢɡɢɪɭɸɳɢɣ ɪɚɫɬɜɨɪ, ɫɨɞɟɪɠɚɳɢɣ 2,5 Ɇ NaCl, 100 ɦɆ EDT A, 10 ɦɆ Tris (ɪɇ 10,0), ɡɚɬɟɦ ɞɨɛɚɜɥɹɥɢ Ɍ ɪɢɬɨɧ ɏ–100. ɉɨ ɨɤɨɧɱɚɧɢɢ ɥɢɡɢɫɚ ɩɪɟɩɚɪɚɬɵ ɩɨɝɪɭɠɚɥɢ ɧɚ 40 ɦɢɧɭɬ ɜ ɚɩɩɚɪɚɬ ɞɥɹ ɷɥɟɤɬɪɨɮɨɪɟɡɚ, ɫɨɞɟɪɠɚɳɢɣ ɳɟɥɨɱɧɨɣ ɛɭɮɟɪ (300 ɦɆ NaOH ɢ 1 ɦɆ EDT A) ɞɥɹ ɪɚɫɤɪɭɱɢɜɚɧɢɹ ɰɟɩɟɣ 73 ȾɇɄ ɢ ɜɵɹɜɥɟɧɢɹ ɳɟɥɨɱɧɨ-ɥɚɛɢɥɶɧɵɯ ɫɚɣɬɨɜ. ɗɥɟɤɬɪɨɮɨɪɟɡ ɩɪɨɜɨɞɢɥɢ ɩɪɢ ɧɚɩɪɹɠɟɧɢɢ 26 ȼ ɢ ɫɢɥɟ ɬɨɤɚ 300 ɦȺ ɜ ɬɟɱɟɧɢɟ 25 ɦɢɧɭɬ. ɉɨɫɥɟ ɷɥɟɤɬɪɨɮɨɪɟɡɚ ɩɪɟɩɚɪɚɬɵ ɨɛɪɚɛɚɬɵɜɚɥɢ ɧɟɣɬɪɚɥɢɡɚɰɢɨɧɧɵɦ ɛɭɮɟɪɨɦ (0,4 Ɇ Tris, ɪɇ 7,5) ɢ ɨɤɪɚɲɢɜɚɥɢ ɪɚɫɬɜɨɪɨɦ ɷɬɢɞɢɭɦɚ ɛɪɨɦɢɞɚ (20 ɦɤɝ /ɦɥ). Ɇɨɞɢɮɢɰɢɪɨɜɚɧɧɵɣ ɦɟɬɨɞ ȾɇɄ-ɤɨɦɟɬ (ɧɟɤɥɟɬɨɱɧɚɹ ɫɢɫɬɟɦɚ). Ⱦɥɹ ɨɰɟɧɤɢ ɭɪɨɜɧɹ ɩɨɜɪɟɠɞɟɧɢɣ ȾɇɄ, ɢɧɞɭɰɢɪɨɜɚɧɧɵɯ ɩɨɪɮɢɪɢɧɨɦ FeT OBut4PyP ɜ ɧɟɤɥɟɬɨɱɧɨɣ ɫɢɫɬɟɦɟ, ɢɫɩɨɥɶɡɨɜɚɥɢ ɳɟɥɨɱɧɭɸ ɜɟɪɫɢɸ ɦɟɬɨɞɚ [8] ɫ ɦɨɞɢɮɢɤɚɰɢɹɦɢ [7]. Ʉɥɟɬɤɢ ɤɪɨɜɢ, ɡɚɤɥɸɱɟɧɧɵɟ ɜ ɫɥɨɣ ɚɝɚɪɨɡɵ, ɨɛɪɚɛɚɬɵɜɚɥɢ FeT OBut4PyP ɩɨɫɥɟ ɩɪɨɰɟɞɭɪɵ ɥɢɡɢɫɚ (ɜ ɤɨɧɰɟɧɬɪɚɰɢɹɯ 10 -7 , 10 -6, 10 -5 M ɜ ɬɟɱɟɧɢɟ 2 ɱɚɫɨɜ ɩɪɢ 370 ɋ). ȼɫɟ ɨɫɬɚɥɶɧɵɟ ɩɪɨɰɟɞɭɪɵ ɨɫɭɳɟɫɬɜɥɹɥɢ ɚɧɚɥɨɝɢɱɧɨ ɬɚɤɨɜɵɦ ɞɥɹ ɤɥɟɬɨɱɧɨɣ ɫɢɫɬɟɦɵ. Ⱥɧɚɥɢɡ ɢɡɨɛɪɚɠɟɧɢɣ ɤɨɦɟɬ . ɂɡɨɛɪɚɠɟɧɢɹ ɤɨɦɟɬ ɪɟɝɢɫɬɪɢɪɨɜɚɥɢ ɫ ɩɨɦɨɳɶɸ ɜɢɞɟɨɤɚɦɟɪɵ (Variocam, PCO, Ƚɟɪɦɚɧɢɹ) ɫ ɩɨɜɵɲɟɧɧɨɣ ɱɭɜɫɬɜɢɬɟɥɶɧɨɫɬɶɸ ɢ ɡɚɩɢɫɵɜɚɥɢ ɜ ɤɨɦɩɶɸɬɟɪ, ɫɧɚɛɠɟɧɧɵɣ ɩɪɨɝɪɚɦɦɨɣ ɚɜɬɨɦɚɬɢɱɟɫɤɨɝɨ ɚɧɚɥɢɡɚ CASP (www.Casp.com). ȼ ɤɚɠɞɨɦ ɜɚɪɢɚɧɬɟ ɚɧɚɥɢɡɢɪɨɜɚɥɢ ɩɨ 150 ɤɥɟɬɨɤ. ɍɪɨɜɟɧɶ ɩɨɜɪɟɠɞɟɧɢɣ ȾɇɄ ɢɡɭɱɚɥɢ ɧɚ ɨɫɧɨɜɟ ɚɜɬɨɦɚɬɢɱɟɫɤɢɯ ɢɡɦɟɪɟɧɢɣ ɩɪɨɰɟɧɬɚ ȾɇɄ ɜ ɯɜɨɫɬɟ, ɦɨɦɟɧɬɚ ɯɜɨɫɬɚ Ɉɥɢɜɚ (MɏɈ), ɨɬɧɨɲɟɧɢɹ ɞɥɢɧɵ ɤɨɦɟɬɵ ɤ ɟɟ ɜɵɫɨɬɟ (L/H). ɋɬɚɬɢɫɬɢɱɟɫɤɢɣ ɚɧɚɥɢɡ ɩɨɥɭɱɟɧɧɵɯ ɪɟɡɭɥɶɬɚɬɨɜ ɩɪɨɜɨɞɢɥɢ ɫ ɩɨɦɨɳɶɸ ɩɪɨɝɪɚɦɦ Microsoft EXCEL ɢ ST AT GRAPHICS Plus 5.1 ɫ ɩɪɢɦɟɧɟɧɟɧɢɟɦ ɧɟɩɚɪɚɦɟɬɪɢɱɟɫɤɨɝɨ ɬɟɫɬɚ Mann-Whitney (U-test). Ɋɟ ɡɭɥɶɬɚɬɵ ɢ ɨɛɫɭɠɞɟ ɧɢɟ . ɉɨɞɪɨɛɧɵɟ ɪɟɡɭɥɶɬɚɬɵ ɢɡɦɟɪɟɧɢɣ % ȾɇɄ ɜ ɯɜɨɫɬɟ, L/H ɢ MɏɈ ɩɨ 150 ɤɥɟɬɤɚɦ ɥɟɣɤɨɰɢɬɨɜ ɤɪɨɜɢ ɩɪɢ ɞɟɣɫɬɜɢɢ FeT OBut4PyP ɜ ɤɨɧɰɟɧɬɪɚɰɢɹɯ 10 -7 , 10 -6 , 10 -5 M ɜ ɤɥɟɬɨɱɧɨɣ ɢ ɧɟɤɥɟɬɨɱɧɨɣ ɫɢɫɬɟɦɚɯ ɩɪɢɜɟɞɟɧɵ ɜ ɬɚɛɥɢɰɟ. Ʉɨɧɰɟɧɬ% ȾɇɄ ɜ ɯɜɨɫɬɟ M ɏɈ L/H ɪɚɰɢɹ ɫɪɟɞɧɟɟ±ɫɬɚɧɫɪɟɞɧɟɟ±ɫɬɚɧɫɪɟɞɧɟɟ±ɫɬɚɧɩɨɪɮɢɪɢɧɚ ɦɟɞɢɚɧɚ ɦɟɞɢɚɧɚ ɦɟɞɢɚɧɚ ɞɚɪɬɧɚɹ ɞɚɪɬɧɚɹ ɞɚɪɬɧɚɹ ,Ɇ ɨɲɢɛɤɚ ɨɲɢɛɤɚ ɨɲɢɛɤɚ ɋɬɚɧɞɚɪɬɧɵɣ ɦɟɬɨɞ ȾɇɄ-ɤɨɦɟɬ (ɤɥɟɬɨɱɧɚɹ ɫɢɫɬɟɦɚ) Ʉɨɧɬɪɨɥɶ 1,16±0,08 0,87 1,07±0,01 1,06 0,51±0,04 0,42 10 -7 4,54±0,28 3,28* 1,21±0,01 1,18* 2,21±0,17 1,73* 10 -6 5,16±0,56 4,02* 1,28±0,02 1,19* 2,52±0,29 1,81* 10 -5 19,61±1,17 15,49* 1,83±0,04 1,72* 20,42±2,16 11,58* Ɇɨɞɢɮɢɰɢɪɨɜɚɧɧɵɣ ɦɟɬɨɞ ȾɇɄ-ɤɨɦɟɬ (ɧɟɤɥɟɬɨɱɧɚɹ ɫɢɫɬɟɦɚ) 10 -7 6,24±0,36 5,35* ɚ 1,32±0,01 1,29*ɚ 4,05±0,28 3,24* ɚ -6 ɚ ɚ 10 24,45±1,06 21,83* 1,81±0,03 1,73* 25,02±1,49 19,23* ɚ -5 10 100% ɪɚɡɪɭɲɟɧɢɟ ȾɇɄ 100% ɪɚɡɪɭɲɟɧɢɟ ȾɇɄ 100% ɪɚɡɪɭɲɟɧɢɟ ȾɇɄ * – ɞɨɫɬɨɜ ɟɪɧɚɹ ɪɚɡɧɢɰɚ ɩɨ ɫɪɚɜɧɟɧɢɸ ɫ ɤɨɧɬɪɨɥɟɦ (p<0,05); ɚ – ɞɨɫɬɨɜ ɟɪɧɚɹ ɪɚɡɧɢɰɚ ɩɨ ɫɪɚɜ ɧɟɧɢɸ ɫ ɤɥɟɬɨɱɧɨɣ ɫɢɫɬɟɦɨɣ (p<0,05). Ɍ ɚɤɢɦ ɨɛɪɚɡɨɦ, FeT OBut4PyP ɜɨ ɜɫɟɯ ɢɡɭɱɟɧɧɵɯ ɤɨɧɰɟɧɬɪɚɰɢɹɯ ɢ ɩɨ ɜɫɟɦ ɢɫɫɥɟɞɭɟɦɵɦ ɩɚɪɚɦɟɬɪɚɦ ɜɵɡɵɜɚɟɬ ɞɨɫɬɨɜɟɪɧɨɟ ɩɨɜɵɲɟɧɢɟ ɭɪɨɜɧɹ ɩɨɜɪɟɠɞɟɧɢɣ ȾɇɄ ɩɨ ɫɪɚɜɧɟɧɢɸ ɫ ɤɨɧɬɪɨɥɟɦ. 74 Ⱦɥɹ ɧɚɝɥɹɞɧɨɫɬɢ ɩɪɢɜɟɞɟɧɵ ɞɢɚɝɪɚɦɦɵ ɷɮɮɟɤɬɨɜ ɩɨ ɩɪɨɰɟɧɬɭ ȾɇɄ ɜ ɯɜɨɫɬɟ ɜ ɤɥɟɬɨɱɧɨɣ (Ⱥ) ɢ ɧɟɤɥɟɬɨɱɧɨɣ (ȼ) ɫɢɫɬɟɦɚɯ ɦɟɬɨɞɚ ȾɇɄ-ɤɨɦɟɬ. ȼȼ 25 120 20 100 % ȾɇɄ ɜ ɯɜ ɨɫɬ ɟ % ȾɇɄ ɜ ɯɜ ɨɫɬ ɟ Ⱥ Ⱥ 15 10 5 0 80 60 40 20 0 Ʉɨɧɬɪɨɥɶ -7 10-7Ɇ 10 Ɇ -6 10-6Ɇ 10 Ɇ -5 1010-5Ɇ Ɇ Ʉɨɧɬɪɨɥɶ 10-7Ɇ 10-7 Ɇ 10-6Ɇ 10-6 Ɇ -5 10-5Ɇ 10 Ɇ ɋɪɚɜɧɟɧɢɟ ɞɟɣɫɬɜɢɹ FeT OBut4PyP ɜ ɤɥɟɬɨɱɧɨɣ ɢ ɧɟɤɥɟɬɨɱɧɨɣ ɫɢɫɬɟɦɚɯ ɩɨɤɚɡɵɜɚɟɬ, ɱɬɨ ɩɨɪɮɢɪɢɧ ɩɨɜɪɟɠɞɚɟɬ ȾɇɄ ɤɚɤ ɩɪɢ ɜɨɡɞɟɣɫɬɜɢɢ ɧɚ ɧɟɩɨɜɪɟɠɞɟɧɧɭɸ ɤɥɟɬɤɭ, ɬɚɤ ɢ ɧɚ ɧɭɤɥɟɨɢɞ ɜɨ ɜɫɟɯ ɢɡɭɱɟɧɧɵɯ ɤɨɧɰɟɧɬɪɚɰɢɹɯ. ɗɬɨ ɨɡɧɚɱɚɟɬ, ɱɬɨ ɩɨɪɮɢɪɢɧ ɫɩɨɫɨɛɟɧ ɩɪɨɧɢɤɚɬɶ ɱɟɪɟɡ ɤɥɟɬɨɱɧɭɸ ɢ ɹɞɟɪɧɭɸ ɦɟɦɛɪɚɧɵ ɢ ɩɪɹɦɨ ɞɟɣɫɬɜɨɜɚɬɶ ɧɚ ȾɇɄ. Ɉɞɧɚɤɨ ɭɪɨɜɟɧɶ ɪɚɡɪɵɜɨɜ ȾɇɄ, ɜɵɡɜɚɧɧɵɯ ɞɟɣɫɬɜɢɟɦ FeT OBut4PyP ɜ ɢɫɫɥɟɞɨɜɚɧɧɵɯ ɤɨɧɰɟɧɬɪɚɰɢɹɯ, ɜ ɧɟɤɥɟɬɨɱɧɨɣ ɫɢɫɬɟɦɟ ɞɨɫɬɨɜɟɪɧɨ ɜɵɲɟ, ɱɟɦ ɩɪɢ ɞɟɣɫɬɜɢɢ ɧɚ ɢɧɬɚɤɬɧɵɟ ɤɥɟɬɤɢ (ɪ<0,05). Ɉɱɟɜɢɞɧɨ, ɤɥɟɬɨɱɧɵɟ ɦɟɦɛɪɚɧɵ ɜ ɨɩɪɟɞɟɥɟɧɧɨɣ ɫɬɟɩɟɧɢ ɡɚɞɟɪɠɢɜɚɸɬ ɩɪɨɧɢɤɧɨɜɟɧɢɟ ɩɨɪɮɢɪɢɧɚ ɜ ɹɞɪɨ ɢ ɬɟɦ ɫɚɦɵɦ ɨɫɥɚɛɥɹɸɬ ɝɟɧɨɬɨɬɨɤɫɢɱɟɫɤɢɣ ɷɮɮɟɤɬ. Ɍ ɚɤɢɦ ɨɛɪɚɡɨɦ, ɩɪɢɦɟɧɟɧɧɵɣ ɩɨɞɯɨɞ ɦɨɠɟɬ ɛɵɬɶ ɪɟɤɨɦɟɧɞɨɜɚɧ ɞɥɹ ɨɰɟɧɤɢ ɝɟɧɨɬɨɤɫɢɱɟɫɤɢɯ ɷɮɮɟɤɬɨɜ ɪɚɡɧɵɯ ɫɨɟɞɢɧɟɧɢɣ ɢ ɫɪɚɜɧɢɬɟɥɶɧɨɝɨ ɚɧɚɥɢɡɚ ɪɨɥɢ ɤɥɟɬɨɱɧɵɯ ɦɟɯɚɧɢɡɦɨɜ ɜ ɮɨɪɦɢɪɨɜɚɧɢɢ ɩɨɜɪɟɠɞɟɧɢɣ ȾɇɄ. ɉɨɫɬɭɩɢɥɚ 10.11.2006 Ʉɚɮɟɞɪɚ ɝɟɧɟɬɢɤɢ ɢ ɰɢ ɬɨɥɨ ɝɢɢ Ʌ ɂ Ɍ ȿɊ Ⱥ Ɍɍ ɊȺ 1. 2. 3. 4. 5. 6. 7. 8. Bonnett R. – Chem. Soc. Rev., 1995, v. 24, p. 19–33. Vicente Mg.H. – Current Med. Chem., Anti-Cancer Agents, 2001, v. 1, p. 175–194. Z alessky V.N., Mikhalkin I.A. – Europ. J. Clin. Pharm. Ther., 1989, v. 21, p. 23–24. Ⱦɚɥɹɧ ȿ. Ȼ. ɋɬɪɭɤɬɭ ɪɨɨɛɪɚɡɨɜ ɚɧɢɟ ɜ ɤɨɦɩɥɟɤɫɚɯ ȾɇɄ–ɥɢɝɚɧɞ. Ɉɛɪɚɬɢɦɨɟ ɢ ɧɟɨɛɪɚɬɢɦɨɟ ɫɜ ɹɡɵɜ ɚɧɢɟ. Ⱥɜ ɬɨɪɟɮ. ɞɢɫɫ . ɧɚ ɫɨɢɫɤɚɧɢɟ ɭ ɱ. ɫɬ. ɞɨɤɬ. ɮɢɡɦɚɬ ɧɚɭ ɤ. ȿɪ., 2002. T ice R.R., Agurell E., Anderson D., Burlinson B., Hartman A., Kobayashi H., Miyamae Y., Royas E., Ryu J.C., Sasaki Y.F. – Environ. Mol. Mutagen, 2000, v. 35, p. 206–221. F airbairn D.V. et al. – Mutation Research, 1995, v. 339, p. 37–59. Kiff e M., Christenand P., Arni P. – Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2003, v. 537, ʋ 2 , p. 151–168. Singh N.P. et al. – Exp. Cell Research, 1988, v. 175, p. 184–191. 75 Î. ê. زð¶²ðÚ²Ü FeT OBut4PyP äàðüÆðÆÜÆ ¶ºÜàîàøêÆÎ ¾üºÎîܺðÆ Ð²ØºØ²î²Î²Ü ²Ü²ÈƼÀ ´ææ²ÚÆÜ ºì àâ ´ææ²ÚÆÜ Ð²Ø²Î²ð¶ºðàôØ ¸ÜÂ-¶Æê²êîÔºðÆ ØºÂà¸àì ² Ù÷á÷ áõÙ ¸ÜÂ-·Çë³ëïÕ»ñÇ Ù»Ãá¹áí μçç³ÛÇÝ ¨ áã μçç³ÛÇÝ Ñ³Ù³Ï³ñ·»ñáõÙ áõëáõÙݳëÇñí»É ¿ Ýáñ FeT OEt4PyP åáñýÇñÇÝÇ ·»ÝáïáùëÇÏáõÃÛáõÝÁ: ºñÏáõ Ùá¹»ÉÝ»ñÇ ½·³ÛáõÝáõÃÛ³Ý Ý»ñϳ۳óí³Í ï³ñμ»ñáõÃÛáõÝÝ»ñÁ ³ñï³Ñ³ÛïáõÙ »Ý Ýñ³Ýó ϳ½Ù³íáñÙ³Ý ï³ñμ»ñ ٳϳñ¹³ÏÝ»ñÁ: ÎÇñ³éí³Í Ùáï»óáõÙÁ ϳñ»ÉÇ ¿ »ñ³ß˳íáñ»É ï³ñμ»ñ ÙdzóáõÃÛáõÝÝ»ñÇ ·»ÝáïáùëÇÏ ¿ý»ÏïÝ»ñÇ ·Ý³Ñ³ïÙ³Ý ¨ ¸ÜÂ-Ç íݳëí³ÍùÝ»ñÇ ³é³ç³óÙ³Ý ¹»åùáõÙ μçç³ÛÇÝ Ù»Ë³ÝǽÙÝ»ñÇ ¹»ñÇ Ñ³Ù»Ù³ï³Ï³Ý ³Ý³ÉÇ½Ç Ñ³Ù³ñ: K. S. MARGARYAN COMPARATIVE ANALYSIS OF GENOTOXIC EFFECTS OF PORPHYRIN FeTOBut4PyP AT CELL AND ACELLULAR SYSTEMS IN COMET ASSAY Su m m ar y The genotoxicity of the new porphyrin FeT OEt4PyP was investigated at cell and acellular systems. Experimental cell and acellular systems used were based on Comet-assay. T he differences in the sensitivity of two models presented are to reflect the different levels of their organisation. T he approach applied may be recommended for the estimation of genotoxic effects of different agents and comparative analysis of role of cellular mechanisms in DNA damage. 76