The work program of the academic discipline Microbiology, Virology and Immunology" For specialty 560001 "General Medicine" CAIMU for the 20232024 academic year The work program for microbiology, virology, immunology is compiled in accordance with the State educational standard of higher professional education 1.Goals of the discipline: Formation of students as widely educated and qualified medical professionals, aware of their place in medicine, capable of analyzing and predicting complex medical problems in the field of microbiology, virology and immunology. To give students modern knowledge about the role of microorganisms in human life and society, about the morphology, physiology and ecology of microorganisms, methods for studying them. Will teach the fundamental principles of their interaction with the human body, the fundamental algorithms of microbiological, virological and immunological methods for diagnosing infectious diseases, and able to propose mechanisms for solving problems in these areas necessary for the acquisition of professional training and the development of subsequent practical activities of a doctor 2.Objectives of the discipline: 1. the formation of widely educated and qualified medical professionals who have the skills to establish the etiological role of microorganisms in normal and pathological conditions, the interaction of the patterns of structure and functioning of a microbial cell and a viral particle. 2. the formation of widely educated and qualified medical professionals with the skills of bacteriological control of the environment, food, compliance with sterilization regimes and supervision of sources of infection in medical and other institutions, and monitoring the sensitivity of microorganisms to antibiotics and other drugs, the state of microbiocenoses of surfaces and body cavities person. 3. microbiological service, its tasks in the health care system and organizational practical forms of solving these problems. 3. The place of the discipline among the main educational program: Basic part of the professional cycle 3. Requirement as a result of studying disciplines: A graduate of specialty 560001 “General Medicine”, in accordance with the goals and objectives of professional activity, must have the following competencies: a) general: OK-4 - is able to analyze economic problems and social processes, use methods of economic relations in the health care system; OK-7 - capable of using control methods; organize the work of the team, find and make responsible management decisions within the framework of their professional competence; OK-8 - is able to carry out its activities taking into account the moral and legal norms accepted in society; comply with the rules of medical ethics, laws and regulations regarding the handling of confidential information; maintain medical confidentiality; b) professional: PK-4 - is able to apply aseptic and antiseptic methods, use medical instruments, knows the technique of caring for sick adults and children; PC-6 - is able to apply modern information on population health indicators at the health care facility level; PC-7 - carry out preventive measures to prevent infectious, parasitic and noninfectious diseases, carry out sanitary educational work on hygienic issues; PK-10 is capable of carrying out anti-epidemic measures, protecting the population in areas of particularly dangerous infection, in case of deterioration of the radiological situation and natural disasters; Know: - current trends and problems in medical microbiology and other branches of medicine; - methods of generalization, analysis, perception of information in the field of microbiology and other branches of medicine; - basic patterns and trends in the development of the infectious process; - know the key concepts of microbiology and how to use them in solving microbiological and professional problems; - basic modern research and methods of immuno-chemo- and antiseptic therapy and prevention of infectious diseases; - Theoretical foundations of computer science in medical microbiological systems, the use of computer information systems and periodicals. - Solving clinical problem-situational tasks; independent preparation of oral abstract reports on program issues with their subsequent discussion. Be able to: - identify the main factors and trends in the development of medical microbiology; - critically evaluate various theoretical approaches existing in the field of medical microbiology; - apply theoretical knowledge to analyze current problems in medical microbiology; - independently select and analyze pathological materials for laboratory research, apply the results obtained for the diagnosis of infectious diseases - use equipment, nutrient media, chemical reagents and conduct microbiological experiments; - work with original scientific medical texts, adequately interpret medical texts of various doctrinal orientations - use educational, scientific, popular science literature, the Internet and an educational portal for professional activities. - collect, process and summarize information in the medical field and apply basic theoretical and methodological approaches. Own: - culture of laboratory thinking in the field of medical microbiology, understanding of current microbiological realities and challenges of globalization; - methods of microbiological interaction in public medicine, characterized by the presence of significant microbiological and general medical differences; - skills in scientific research and analysis of microbiological problems and processes related to medical microbiology and other branches of medicine; - basic theoretical and methodological approaches when approaching medical microbiology and other branches of medicine in the field of microbiological forecasting 4.Scope of discipline and types of academic work 4.1.Full-time education Total Type of educational work hours Classroom lessons (total) 240 Including: Lectures Practical exercises (PL) Seminars (C) Independent work (total) Total Type of intermediate certification (test, exam) Semesters 2 3 90 150 15 30 30 45 45 90 75 150 Exam Exam 5. Thematic plan of lectures in the discipline “Microbiology, Virology and Immunology” 2nd year III – semester specialty “General Medicine” for the 2023-2024 academic year. № 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Lesson topics Volum e hours Subject and tasks of microbiology, immunology and virology. 2 Research methods in microbiology. 2 Basic principles of classification of microorganisms. Morphology of 2 bacteria. The structure of a bacterial cell and its elements. 2 Physiology of bacteria. 2 Genetic engineering in medical microbiology. Genetics of microorganisms. Organization of the genetic apparatus in bacteria and viruses. Genetic engineering in medical microbiology. Microbiological basis of chemotherapy of infectious diseases. Chemotherapy drugs. Antibiotics. Drug resistance of bacteria. General virology Pathogenic fungi 1, 2 Pathogenic protozoa 1, Pathogenic protozoa 2 Causative agents of purulent inflammatory processes. Gram-positive cocci 2 2 2 2 2 2 2 2 2 2 30 Gram-negative cocci Total: 6. Schedule of lectures in the discipline “Microbiology, Virology and Immunology” 2nd year IV – semester specialty “General Medicine” for the 2022-2023 academic year № 1 2 3 Lesson topics Pathogens of diphtheria, whooping cough and parawhooping cough Pathogens of tuberculosis and leprosy. Causative agents of bacterial intestinal infections: Salmonella, Enterobacteriaceae, Escherichia, Shigella Volume hours 2 2 2 4 5 6 7 8 9 10 11 12 13 14 15 Causative agents of cholera. Causative agents of botulism. Pathogens of tularemia Pathogens of zoonotic infections - anthrax. The causative agents of zoonotic infections are brucellosis. Causative agents of anaerobic infections. Causative agents of gas gangrene, tetanus Pathogens of pathogenic rickettsia. RNA viruses: Filoviruses. Coronaviruses. Caliciviruses. Reoviruses RNA viruses: Retroviruses. Pathogens of HIV infection. DNA viruses: Herpes viruses. Papoviruses.Adenoviruses DNA viruses: Poxviruses. Vapor viruses. Hepadnoviruses. RNA viruses: Orthomyxoviruses. Paramyxoviruses. Picarnoviruses. 2 2 2 2 2 2 Total: 30 2 2 2 2 2 2 7. Title of the topic of practical classes in the discipline “Microbiology, Virology and Immunology” 2nd year III – semester specialty “General Medicine” for the 2022-2023 academic year № 1 2 3 4 Lesson topics Subject and tasks of microbiology, immunology and virology. History of the development of microbiology. Design and equipment of a laboratory tank, safety precautions and operating rules. Research methods in microbiology. Light microscope and rules for working with it. Microscopic research methods. Basic principles of classification of microorganisms. Morphology of bacteria. Dyes of microorganisms. Preparation of a smear. Simple ways to stain bacteria. The structure of a bacterial cell and its elements. Sporulation in bacteria. Inclusion of microbial cells, volutin grains. Motility of bacteria. Sophisticated methods for staining microbes. Technique and essence 6 7 Physiology of bacteria. Metabolism of microorganisms. Mechanism of bacterial nutrition. 8 Nutrient media, their classification. Principle of preparation of nutrient media. 9 Growth and reproduction of microbes. Principles of microorganism cultivation 10 Sterilization and disinfection. 11 Bacterial enzymes. Determination of the type of bacteria by enzymatic activity. 12 Bacterial pigments and their significance. Luminous and aromaforming microorganisms. 5 Volume hours 2 2 2 2 2 2 2 2 2 2 2 2 13 Genetics of microorganisms. Organization of the genetic apparatus in bacteria and viruses. Genetic recombination. 14 Methods for selection of recombinants. Bacterial plasmids. Genetic engineering in medical microbiology. 15 Biological research method. 16 Microbiological basis of chemotherapy of infectious diseases. Chemotherapy drugs. Antibiotics. 17 Drug resistance of bacteria. Determination of bacterial sensitivity to antibiotics. 18 General virology. Classification of viruses. Morphology and structural structure of viruses. Virus-cell interactions. Bacteriophage viruses, morphological features, significance of bacteriophages. 19 Virological research methods. Methods for growing and displaying viruses 20 Medical mycology, taxonomy of fungi, general characteristics of pathogenic fungi. Principles of classification. Morphology, mushroom cultivation. 21 Disease caused by fungi. Superficial and deep mycoses morphology, cultural properties. 22 Pathogenic protozoa. Amoeba. Giardia. Leishmania. Trichomonas. Trypanosomes. 23 Plasmodium malaria. Toxoplasma. The causative agent of balantidiasis. 2 2 2 2 2 2 2 2 2 2 1 45 Total: 8. Title of the topic of practical classes in the discipline “Microbiology, Virology and Immunology” 2nd year IV – semester specialty “General Medicine”. for the 2022-2023 academic year № 1 2 3 4 5 6 7 8 9 10 11 12 Lesson topics Causative agents of diphtheria. Causative agents of whooping cough and parawhooping cough. Pathogens of tuberculosis and leprosy. Pathogens of actinomycosis. Causative agents of bacterial intestinal infections: salmonella. The causative agents of typhoid and paratyphoid A and B. Bacteriological and serological diagnosis of typhoid-paratyphoid fever, ethereal bacteria. Causative agents of bacterial intestinal infections: enterobacteriaceae, Escherichia, Shigella. Causative agents of bacterial intestinal infections: Klebsiella, Protea. Causative agents of cholera. Causative agents of cholera. Pathogens of plague. Pathogens of tularemia Volume hours 2 2 2 2 2 2 2 2 2 2 2 2 13 14 15 16 17 18 19 20 21 22 23 Pathogens of zoonotic infections - anthrax. The causative agents of zoonotic infections are brucellosis. Causative agents of anaerobic infections. Causative agents of gas gangrene, tetanus Causative agents of botulism. Causative agents of pathogenic rickettsia: causative agents of typhus, BrillZinsser disease, Q fever, Microbiological diagnosis of rickettsia Pathogens of pathogenic spirochetes: Borrelia - causative agents of relapsing fever. Leptospira is the causative agent of leptospirosis. Treponema pallidum is the causative agent of syphilis. Microbiological diagnosis of spirochetes. DNA viruses: Herpes viruses. Papoviruses.Adenoviruses DNA viruses: Poxviruses. Vapor viruses. Hepadnoviruses. RNA viruses: Orthomyxoviruses. Paramyxoviruses. Picarnoviruses. Total: 2 2 2 2 2 2 2 2 2 2 1 45 9.Independent work of students (SWS) in the discipline “Microbiology, virology, immunology” for students majoring in general medicine, 2nd year, 3rd semester for the 2022-2023 academic year. № 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 Topic name Modern principles applied to bacterial taxonomy Report form 1. Abstract 2.Presentation 3.report Modern achievements of biotechnology. Transgenic, 1. Abstract microorganisms, plants, animals. 2.Presentation 3.report Modern achievements of biotechnology. Transgenic, 1. Abstract microorganisms, plants, animals. 2.Presentation 3.report Heat shock proteins in bacteria. 1. Abstract 2.Presentation 3.report Bacterial toxins. Properties. Application in medicine. 1. Abstract 2.Presentation 3.report History of vaccination. 1. Abstract 2.Presentation 3.report Intraspecific methods 1. Abstract 2.Presentation identification of bacteria.. 3.report Gene therapy. Problem, prospects for application. 1. Abstract 2.Presentation 3.report Microorganisms as symbiotic partners.. 1. Abstract 2.Presentation 3.report Evolution of microorganisms. 1. Abstract 2.Presentation 3.report Mechanisms of action of antimicrobial agents. 1. Abstract 2.Presentation 3.report Compatibility of antibiotics with other drugs. 1. Abstract 2.Presentation 3.report The role of viruses and plasmids in ontogenesis. 1. Abstract 2.Presentation 3.report Mechanisms of resistance to antibacterial agents. 1. Abstract 2.Presentation 3.report Bacteriophage viruses, morphological features, 1. Abstract significance of bacteriophages 2.Presentation 3.report Pathogens of systemic or deep mycoses. 1. Abstract Number of hour 2 3 2 3 3 2 3 3 2 3 3 3 3 3 3 3 17 Causative agents of subcutaneous and epidermal superficial mycoses. Conditionally pathogenic fungi and mycoses caused by them. 18 Ureaplasma urealyticaum. Biological properties, clinical significance, diagnosis of human diseases.. 19 Staphylococcus pylori. Friend or foe... 20 Monitoring of pathogens of purulent-inflammatory diseases in the hospital. № 1 2 3 4 2.Presentation 3.report 1. Abstract 2.Presentation 3.report 1. Abstract 2.Presentation 3.report 1. Abstract 2.Presentation 3.report 1. Abstract 2.Presentation 3.report 1. Abstract 2.Presentation 3.report 1. Abstract 2.Presentation 3.report 21 Differential principles of streptococcal species 22 Pathogens not gonorrheal urethritis – chlamydia, 23 Pathogens 1. Abstract non-gonorrheal 2.Presentation urethritis 3.report – mycoplasma Total 10.Independent work of students (SRS) 2nd year 4th semester Topic name Determination of diphtheria toxigenicity in vitro Report form 1. Abstract 2. Presentation 3. report Bacteriological and serological studies of whooping 1. Abstract cough and parapertussis 2. Presentation 3. report Microbiological 1. Abstract diagnosis of leprosy. 2. Presentation 3. report Pathogens of tuberculosis. 1. Abstract 2. Presentation 3. report Number of hours 2 3 3 2 3 3 2 2 2 2 2 60 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 Chronic brucellosis. Modern diagnosis and treatment. approaches to 1. Abstract 2. Presentation 3. report Pathogens of typhoid fever and paratyphoid fever. 1. Abstract 2. Presentation 3. report Causative agents of salmonellosis. 1. Abstract 2. Presentation 3. report Causative agents of dysentery. 1. Abstract 2. Presentation 3. report Pathogens of cholera, Vibrio 1. Abstract paragemolyticus 2. Presentation 3. report Microbiological 1. Abstract diagnostics Y. 2. Presentation enterocolitica 3. report Pathogens of gas 1. Abstract gangrene – Clostridium novy, Clostridium 2. Presentation oedematiens, Clostridium septicum. 3. report Particularly dangerous infections. 1. Abstract 2. Presentation 3. report Pathogens of cholera, Vibrio 1. Abstract paragemolyticus 2. Presentation 3. report Brill-Zinsser disease. 1. Abstract 2. Presentation 3. report Differentiation of epidemic and endemic typhus. 1. Abstract 2. Presentation 3. report Other treponematoses: yaws, pinta, Bejel. 1. Abstract 2. Presentation 3. report Lyme disease. 1. Abstract 2. Presentation 3. report Microbiological 1. Abstract glanders diagnosis 2. Presentation 3. report Microbiological 1. Abstract diagnostics 2. Presentation 3 2 2 2 2 2 3 2 2 2 2 2 2 2 2 melioidosis 20 Pathogens nocardiosis. 21 Pathogens yaws, pint. 22 Adenoviruses and rhinoviruses 23 Reviruses and RS - viruses 24 Norwalk virus 25 Caliciviruses 26 The causative agents of Kuru disease, GerstmannStraussler-Scheinker syndrome. Hereditary fatal insomnia Causative agents of Scrapie disease (from the English scrape - scrape), bovine spongiform encephalopathy, Creutzfeldt-Jakob disease (CJD) Natural killer cells and proteins of acute phases. Humoral nonspecific factors protection. The role of immunoglobulin classes in immunity newborns due to their accumulation in organisms of mother and fetus. Applied immunology. Molecular biological methods: NA hybridization, PCR, DNA scanning. Vaccines, immune serums, immunoglobulins. Vaccination calendar. 27 28 29 30 31 32 Immunoserological and immunocytological tests for the diagnosis of allergies. 33 Delayed hypersensitivity reaction 3. report 1. Abstract 2. Presentation 3. report 1. Abstract 2. Presentation 3. report 1. Abstract 2. Presentation 3. report 1. Abstract 2. Presentation 3. report 1. Abstract 2. Presentation 3. report 1. Abstract 2. Presentation 3. report 1. Abstract 2. Presentation 3. report 1. Abstract 2. Presentation 3. report 1. Abstract 2. Presentation 3. report 1. Abstract 2. Presentation 3. report 1. Abstract 2. Presentation 3. report 1. Abstract 2. Presentation 3. report 1. Abstract 2. Presentation 3. report 1. Abstract 2. Presentation 3. report 2 2 2 2 2 2 2 2 2 2 3 2 2 2 34 Invasiveness of viruses. Genetic control factors of pathogenicity and toxigenicity. 35 Evolution of microbial parasitism and origin of pathogenic microorganisms. 1. Abstract 2. Presentation 3. report 2 1. Abstract 2. Presentation 3. report 2 Total: 75 11.Sections/topics of the discipline and interdisciplinary connections with the provided (subsequent) disciplines No. of sections/topics of this discipline № Name of the provided (subsequent) required for studying the provided п/п disciplines (subsequent) disciplines 1-6 1-12 12-22 12-30 1. medical biology, genetics and + + parasitology 2. histology, embryology, cytology + 3. infectious diseases + + 4. normal physiology + 5. epidemiology + + 6. pathological anatomy + 7. general hygiene + + 12. Material and technical support of the discipline: 12.1.Textbooks, teaching aids, methodological instructions, lecture notes, practical materials, reference books, etc. 12.2. TSO № Topic name п/п Rules for working in bacteriological laboratories. 1. Ultrastructure of a bacterial cell 2. Ultrastructure of a bacterial cell 3. Spirochetes 4. Rickettsia, chlamydia, viruses 5. Actinomycetes 6. Methods for isolating pure cultures of aerobic 7. bacteria Methods for isolating pure cultures 8. Methods for isolating pure cultures 9. Видео слайды + + + + + + + + Плакат Схем ы ы + + + + + + + + + 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23 24 25 26 Prevalence of microbes in nature The effect of environmental factors on microorganisms Bacteriophages Doctrine of infection Pathogens of especially dangerous and zoonotic infections Pathogens of zoonotic diseases Rickettsia. Rickettsia Clostridia. Clostridium Escherichia coli. E.coli Shigella. Shigella Salmonella. Salmonella typhi, S. paratyphi A and S. Staphylococcus Staphylococcus Streptococci. Streptococcus Neisseria. Neisseria meningitidis Corynebacteria Mycobacteria. Mycobacterium tuberculosis Hepatitis viruses B, D, C, G, F + + + + + + + + + + + + + + + + + + + + + + + + + + + 13. Methodological recommendations for organizing the study of the discipline: the section includes the following subsections: 13.1.Features and order of studying the discipline. This course includes both classroom and independent training. Classroom lessons include: • lectures; • practical lessons. • seminars; In the process of studying, the following types of work are expected to be performed by students: in practical classes: problem solving; performing tests; making reports; extracurricular: doing homework; preparation of reports, abstracts; work with basic and additional literature, work with materials on the Internet. 14.Educational technologies When studying the discipline, traditional forms of teaching are used using a module-rating system of training and control of student knowledge. Information technologies are widely used: multimedia presentation of lectures, student work in the classroom of the department, electronic textbooks on the discipline. Active and interactive forms of conducting classes The following forms of conducting classes are widely used: - work in small groups; - analysis of a specific situation; - discussion; - defense of an abstract with a multimedia presentation. 15.List of basic and additional educational literature necessary for mastering the discipline Main literature Year, place п/ № Name Аuthors) 1. Pozdeev O.K. publications M.: GEOTARMedia, 2010.- *Tsarev V.N. M.: Practical medicine, 2017.- Medical microbiology. Textbook Microbiology, virology and 2. immunology. Textbook Medical and sanitary microbiology. Educational 3. allowance 4. Immunology. Textbook Vorobiev A.A. Khaitov R.M. Laboratory workshop works with illustrated situational tasks for microbiology, immunology 5. and virology * . Vorobiev A.A General medical *Goryachkina 6. virology. Tutorial N.S. additional literature п №/ Name Medical microbiology. 1. Textbook Medical microbiology. 2. Textbook M.: Academy, 2015.M.: GEOTARMedia, 2014.- M.: MIA, 2016.Rostov n/d.: Phoenix, 2018.- Pozdeev O.K. Year, place publications M.: GEOTARMedia, 2010 Pozdeev O.K. M.: GEOTARMedia, 2010 Authors) Microbiology. Textbook M.: Medicine, 3 MZRF * Vorobiev A.A 2015 Fundamentals of microbiology, virology and immunology. 4. Textbook MORPH *. Vorobiev A.A Мастерство, 2013 М.: ГЭОТАР МЕДИЦИНА, 2016 5. Visual immunology J. Playfair Microbiology, immunology, virology. Methodical guidelines for practical *Koltsov I.P. classes for medical students, *Nesterenko ped. and ostomy faculties. In 3 L.Ya. Khabarovsk: 6. Parts DVSMU, 2018 Microbiology, immunology, virology. Guide for independent work for medical students, ped. and *Koltsov I.P. ostomy *Nesterenko Khabarovsk: 7. faculties. In 3 parts L.Ya. DVSMU, 2018 Viral diseases person with fecal-oral by transfer. Guide to practical training for medical students, ped. and *Kogut E.P. ostomy *Koltsov I.P. Khabarovsk: 8. faculties Ch.11 DVSMU, 2017 Viral diseases man with air by droplet transmission. Guide to practical classes for medical students, *Kogut E.P. Khabarovsk: 9. ped. and ostomy faculties Ch.10 *Koltsov I.P. DVSMU, 2013 Introduction to Microbiology oral cavity. Benefit for *Koltsov I.P. Khabarovsk: 10. Students *Nesterenko L.Ya DVSMU, 2014 16. List of resources of the information and telecommunications network "Internet" (hereinafter referred to as the "Internet") necessary for mastering the discipline 1. http: // www. ramld.ru 2. http: // www.antibiotic.ru 3. http: // cmr.asm.org 4. http: // mmbr.asm.org 5. http: // www.inbi.ras.ru/ pmb.html 6. http: // www.rusmedserv.com/ microbiology/ articles 7. http: // www.medlit.ru medrus/vop.vir.htm 8. http:// www.mycology.ru