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e-mail: vijailakshmi@rediff.com
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Dexq_\u_keh\ZZf_[hpb^gh_^_ckl\b_Entamoeba histolyticaZf_[bZake_ihcdbrdbLobophytum pauciflorum.
Activity of soft coral Lobophytum pauciflorum extract against Entamoeba histolytica in vitro and in vivo.
S. Ghoshal1, V. Lakshmi2 (1Division of Microbiology and 2Division of Chemistry, Central Drug Research Institute,
Lucknow-226001, India)
Methanol extracts of the marine coral Lobophytum pauciflorum showed in vitro amoebicidal activity against
trophozoites of Entamoeba histolytica and effected 85% cures of caecal amoebiasis in rats. The activity was localized in
the n-butanol fraction, from which three pure compounds were isolated. (Biologiya Morya, Vladivostok, 2004, vol. 30,
no. 6, pp. 483–485).
Key words: amoebicidal activity, Entamoeba histolytica, caecal amoebiasis, Lobophytum pauciflorum.
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Anjaneyulu V., Babu B.H $ QHZ WULK\GUR[\ VWHURO PHWK\OFKROHVWHQH UWULRO IURP D VRIW FRUDO RI
Lobophytum species of the Indian Ocean // Indian J. Chem.
1992. Vol. 31. P. 708–710.
Beltran F. Epidemiologica de las infeciones on Entamoeba histolytica. Sect.VIII. Protozoan diseases // Proc. 4th Int. Congr.
on Tropical Medicine and Malaria, Washington. 1948.
Vol. 2. P. 1056.
Cedillos R.A., Reeves R.E., Swartzwielder J.C. Influence of added
cholesterol and dehydrocholesterol upon the multiplication
of Entamoeba histolytica in MS-F medium // Exp. Parasitol.
1961. Vol. 11. P. 305–310.
;BHEH=BYFHJYlhf‹
485
Das S.R. A novel and rapid method for in vitro testing of antiamoebic agents against aerobic and anaerobic amoebae
growing axenically or with bacteria // Curr. Sci. 1975.
Vol. 44. P. 463–464.
Diamond L.S., Harlow D.R., Cunnick C.C. A new medium for the
axenic cultivation of Entamoeba histolytica and other Entamoeba // Trans. Roy. Soc. Trop. Med. Hyg. 1978. Vol. 72.
P. 431–432.
Edrada R.A., Proksh P., Wray V. et al. Four new bioactive lobane
diterpenes of the soft coral Lobophytum pauciflorum from
Mindoro, Philippines // J. Nat. Prod. 1998. Vol. 61. P. 358–
361.
Krishna Prasad B.N., Bansal I. Interrelationship between faecal
pH and susceptibility to Entamoeba histolytica infection of
rats // Trans. Roy. Soc. Trop. Med. Hyg. 1983. Vol. 77.
P. 271–274.
Leitch G.J. Intestinal luminal and mucosal microclimate H– and
NH+ concentration as factors in the etiology of experimental
amoebiasis // Am. J. Trop. Med. Hyg. 1988. Vol. 38.
P. 480–496.
Neal R.A. Some observations on the variations of virulence and
response to chemotherapy of strains of Entamoeba histolytica // Trans. Roy. Soc. Trop. Med. Hyg. 1951. Vol. 44.
P. 439–452.
Raju B.L., Subaraju G.V., Rao C.B., Trimurtulu G. Two new
FU\hJHQDWHGOREDQHVIURPVRIWFRUDORILobophytum species
of the Andaman and Nicobar coasts // J. Nat. Prod. 1993.
Vol. 56. P. 961–966.
Robinson G.R. Laboratory cultivation of some human parasitic
amoeba // J. Gen. Microbiol. 1968. Vol. 53. P. 19–29.
Stanley S.L. Jr. Amoebiasis // Lancet. 2003. Vol. 361. P. 1025–
1034.
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