Занятие 1. Химическое строение белка. Методы исследования

1.
.
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;
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1.
2.
3.
4.
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5.
-
N-
(
,
,
).
6.
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:
1.
1.
1.
1
2
3
4
5
6
7
8
9
10
(
)
2.
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,
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,
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,
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3.
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4.
5.
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164
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6.
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7.
:
8.
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;
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;
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,
:
1
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1.
.
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,
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(II)
(
)
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-
.
: 5
3
10%
NaOH 1
10%
CuSO4,
.
: ______________________________________________________________
_______________________________________________________________________________
_______________________________________________________________________________
:
: ___________________________________________________________________
_______________________________________________________________________________
2.
.
.
,
:
,
,
.
,
.
.
: 5
2-3
HNO3,
.
: ______________________________________________________________
_______________________________________________________________________________
_______________________________________________________________________________
1,5
10%
NaOH
.
: ______________________________________________________________
_______________________________________________________________________________
_______________________________________________________________________________
:
: ___________________________________________________________________
_______________________________________________________________________________
2
3.
.
.
@
,
.
:
5
5
0,6
5%
.
.
.
: ______________________________________________________________
_______________________________________________________________________________
_______________________________________________________________________________
: ___________________________________________________________________
_______________________________________________________________________________
_______________________________________________________________________________
1.
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,
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–
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–
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2.
26000
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;
–
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)
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:
,
,
.
Halobacterium halobium
,
4,5
(
. 1).
( %)
,
,
,
,
,
.
,
110
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2
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1.
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3
2.
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3.
4.
(
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1.
2.
2.
,
2.
pH
pI
pH = 3,0
pH = 5,6
:
pH = 8,0
–
–
–
3.
,
,
,
,
pH = 6,2.
(
,
,
pH 6,2
)
,
.
4.
:
1 – 12
,
2 – 19
Rf
;
,
0,48;
– 0,32;
– 40
3 – 25
;
;
.
–
:
– 0,38.
1.
(
)
.
,
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4
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@
:
.
.
5
1–2
1%
CuSO4.
: ______________________________________________________________
_______________________________________________________________________________
_______________________________________________________________________________
1
,
2–5
10%
CuSO4.
: ______________________________________________________________
_______________________________________________________________________________
_______________________________________________________________________________
.
(
).
5
(
45°,
,
)
.
,
.
: ______________________________________________________________
_______________________________________________________________________________
_______________________________________________________________________________
.
.
5
96%
.
: ______________________________________________________________
_______________________________________________________________________________
_______________________________________________________________________________
.
: ___________________________________________________________________
_______________________________________________________________________________
2.
.
.
–
,
,
= 7,
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,
,
0,
).
.
.
:
.
5
96%
1%
4,75
1,0
1,5
0,5
3,4
1,0
1,5
0,5
@
5,7
1,0
1,5
0,5
: ___________________________________________________________________
_______________________________________________________________________________
1.
pH 6,5
.
,
,
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,
:
)
)
;
.
.
2.
,
39%,
3(
.
100%.
25000) – 63%,
15000)
2 (
35000)
4 ( 20000) – 81%
1 (
1.
,
2
3.
3.
(
.
3).
.
3
pI
151000
150000
37100
-
4,4
6,3
5,2
3
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7.
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8.
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9.
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10.
4.
4.
1.
E7
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Fe2+
Fe3+.
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2.
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2
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2,3-
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2,3-
2
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3
4.
2,3?
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?
7